| 50 | | Create a summary VCF per batch: |
| 51 | | -ss : remove sample details! |
| 52 | | -fv PASS : keep only high quality variant calls that pass all filters applied in NextGene. |
| 53 | | Just to be sure: variants should already have been filtered on PASS only in a previous step, |
| 54 | | so this should be redundant here... |
| 55 | | -si : remove all INFO subfields except for INFO:AN and INFO:AC. |
| 56 | | INFO:AN and INFO:AC were automatically updated by vcf-merge, |
| 57 | | but the others were not and may contain erroneous annotation |
| 58 | | that cause vcf-validator to complain the created VCF is not valid. |
| 59 | | -ll : specifies log level, e.g. INFO |
| 60 | | |
| | 52 | {{{ |
| | 53 | # |
| | 54 | # Create a summary VCF per batch: |
| | 55 | # -ss : remove sample details! |
| | 56 | # -fv PASS : keep only high quality variant calls that pass all filters applied in NextGene. |
| | 57 | # Just to be sure: variants should already have been filtered on PASS only in a previous step, |
| | 58 | # so this should be redundant here... |
| | 59 | # -si : remove all INFO subfields except for INFO:AN and INFO:AC. |
| | 60 | # INFO:AN and INFO:AC were automatically updated by vcf-merge, |
| | 61 | # but the others were not and may contain erroneous annotation |
| | 62 | # that cause vcf-validator to complain the created VCF is not valid. |
| | 63 | # |
| | 64 | }}} |