50 | | Create a summary VCF per batch: |
51 | | -ss : remove sample details! |
52 | | -fv PASS : keep only high quality variant calls that pass all filters applied in NextGene. |
53 | | Just to be sure: variants should already have been filtered on PASS only in a previous step, |
54 | | so this should be redundant here... |
55 | | -si : remove all INFO subfields except for INFO:AN and INFO:AC. |
56 | | INFO:AN and INFO:AC were automatically updated by vcf-merge, |
57 | | but the others were not and may contain erroneous annotation |
58 | | that cause vcf-validator to complain the created VCF is not valid. |
59 | | -ll : specifies log level, e.g. INFO |
60 | | |
| 52 | {{{ |
| 53 | # |
| 54 | # Create a summary VCF per batch: |
| 55 | # -ss : remove sample details! |
| 56 | # -fv PASS : keep only high quality variant calls that pass all filters applied in NextGene. |
| 57 | # Just to be sure: variants should already have been filtered on PASS only in a previous step, |
| 58 | # so this should be redundant here... |
| 59 | # -si : remove all INFO subfields except for INFO:AN and INFO:AC. |
| 60 | # INFO:AN and INFO:AC were automatically updated by vcf-merge, |
| 61 | # but the others were not and may contain erroneous annotation |
| 62 | # that cause vcf-validator to complain the created VCF is not valid. |
| 63 | # |
| 64 | }}} |