wiki:Courses/ComputationalMolecularBiologyResearch2016

Version 16 (modified by Morris Swertz, 8 years ago) (diff)

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Computational Molecular Biology Research

Table of Contents

  1. Course Overview
  2. Rating
  3. Projects
    1. Increasing diagnostic yield of fast whole genome diagnostics using …
    2. Improved quantification for RNA sequencing - what combination of …
    3. Methods/tools for detection of fusion gene transcripts in RNA sequencing
    4. Single cell RNA sequencing quantification
    5. Improved detection of regulatory transcription factor binding sites in …
  4. Course Schedule
    1. Venue
    2. Regular events
    3. Week 1
    4. Weeks 2-5
    5. Week 6
    6. Contact

Course Overview

This research course demonstrates the in silico data analysis used for genetics research at the RUG and UMCG. The emphasis of the research topics is on finding better methods for the analysis of high throughput data generated using technologies like Next Generation Sequencing (both DNA and RNA), Gene expression arrays, Genome wide association studies (GWAS), etc.

In the first week the research topics will be introduced and a minimal introduction for scripting in Bash and R will be provided. At the end of the first week the students will choose a research project to work on under supervision of a PhD-student or Postdoc. In weeks 2-5 the in silico experiments are performed. The 6th and last week is reserved for writing a report, preparing a presentation and presenting the results to the other in the department.

Rating

Students will be rated as follows:

  • 50% Code + data produced during practical work
  • 30% Report
  • 20% Presentation

Projects

Increasing diagnostic yield of fast whole genome diagnostics using MtDNA for new borns on the Neonatal Intensive Care Unit (NICU)

  1. Improved variant calling for MtDNA - Pieter / Gerben

Improved quantification for RNA sequencing - what combination of methods should we use?

  1. Improved quantification for RNA sequencing - Niek / Freerk

Methods/tools for detection of fusion gene transcripts in RNA sequencing

  1. Analysis of trans-spliced transcripts in RNA seq data - Lennart / Birgit / Analyse team

Single cell RNA sequencing quantification

  1. Single cell RNA seq quantification - Gerben / Pieter van der Vlies / Iris? [waar komt de data vandaan?]

Improved detection of regulatory transcription factor binding sites in non-coding DNA by merging sequence (context) with in vivo DNA accessibility data

  1. Improved DNA Motif finding - Iris / Pieter

Course Schedule

Venue

Course will be organized at or near the UMCG Genetics department, which is located on the 5th floor of the European Research Institute for the Biology of Aging - ERIBA building plus the complete 5th and partial 6th floor of the adjacent Dentistry building. The address for both buildings is 

Antonius Deusinglaan 1
9713 AV Groningen
Building Room Name
3226 302 ERIBA building, meeting room, 3rd floor, in the corner and next to auditorium
3226 314 ERIBA building, auditorium, 3rd floor
3211 519 Dentistry building, meeting room without a name opposite the secretary, 5th floor, opposite the secretary and near elevators
3211 501 Dentistry building, meeting room 1, 5th floor, end of corridor
3211 flex desks Dentistry building, 5th floor
3226 flex desks ERIBA building, 5th floor

Regular events

Day Time What Where
Mondays 09:15 - 09:30 Course SCRUM in weeks 2-6 TBA instant meeting room 3211 5th floor
Mondays 12:00 - 13:00 Research meeting (Wijmenga/Weersma/Franke/Swertz groups) 3226-314 ERIBA auditorium
Tuesdays 08:45 - 10:00 Research meeting (Entire genetics dept.) 3226-314 ERIBA Auditorium
Tuesdays 10:15 - 10:30 Course SCRUM in weeks 2-6 TBA instant meeting room 3211 5th floor
Wednesdays 09:15 - 09:30 Course SCRUM in weeks 2-6 TBA instant meeting room 3211 5th floor
Thursdays 09:15 - 09:30 Course SCRUM in weeks 2-6 TBA instant meeting room 3211 5th floor
Thursdays 12:00 - 13:00 Literature club (Wijmenga/Weersma/Franke groups) 3226-314 ERIBA auditorium
Fridays 09:15 - 09:30 Course SCRUM in weeks 2-6 TBA instant meeting room 3211 5th floor

Week 1

Monday 01-02-2016

When What Where
09:15 - 09:45 Course kick off (Pieter Neerincx) TBA
09:45 - 10:00 Break
10:00 - 10:45 Lecture: Next Generation Sequencing (Morris Swertz) TBA
10:45 - 11:00 Break
11:00 - 11:45 Project intro: (Birgit Sikkema-Raddatz / Lennart Johansson)TBA
11:45 - 12:00 Break
12:00 - 13:00 Research lunch meeting (Wijmenga/Weersma/Franke/Swertz groups) 3226-314 ERIBA Auditorium
13:00 - 13:45 Bash scripting intro part I (Lennart Johansson) TBA
13:45 - 14:00 Break
14:00 - 17:00 Bash scripting tutorials TBA

Tuesday 02-02-2016

When What Where
08:45 - 09:45 Research meeting (Entire genetics dept.) 3226-314 ERIBA Auditorium
09:45 - 10:00 Break
10:00 - 10:45 Lecture: Experimental Design (Lude Franke) TBA
10:45 - 11:00 Break
11:00 - 11:45 Lecture: GWAS (Lude Franke) TBA
12:00 - 13:00 Lunch break
13:00 - 13:45 Bash scripting intro part II (Lennart Johansson) TBA
13:45 - 14:00 Break
14:00 - 17:00 Bash scripting tutorials TBA

Wednesday 03-02-2016

When What Where
09:00 - 12:00 R-turorial part I (Annique Claringbould) TBA
12:00 - 13:00 Lunch break
13:00 - 17:00 R-turorial part II (Annique Claringbould) TBA

Thursday 04-02-2016

When What Where
09:00 - 09:45 Project intro: Quantification for RNA seq (Niek de Klein) TBA
09:45 - 10:00 Break
10:00 - 10:45 Project intro: mtDNA analysis (Pieter Neerincx) TBA
10:45 - 11:00 Break
11:00 - 11:45 Project intro: DNA Motif finding (Iris Jonkers) TBA
12:00 - 13:00 Lunch break
13:00 - 13:45 Lecture: Gene Expression (Lude Franke) TBA
13:45 - 14:00 Break
14:00 - 14:45 Lecture: Integrating Datasets (Lude Franke) TBA
14:45 - 15:00 Break
15:00 - 15:45 Lecture: Data visualization (Lude Franke) TBA
15:45 - 16:00 Break
16:00 - 16:45 Project intro: Single cell RNA seq (Pieter van der Vlies) TBA
18:00 Deadline: Preferences for project mailed to course coordinator Pieter Neerincx

Friday 05-02-2016

When What Where
09:15 - 09:30 Scrum TBA
09:30 - 12:00 Getting familiar with your project:
Search literature,
Design experiment and
write (validation) plan 		flex desks
12:00 - 13:00 Lunch break
13:00 - 17:00 Getting familiar with your project:
Search literature,
Design experiment and
write (validation) plan 		flex desks

Weeks 2-5

Monday 08-02-2016

When What Where
09:15 - 09:30 Scrum TBA
09:30 - 12:00 Finish experimental design / (validation) plan flex desks
12:00 - 13:00 Research lunch meeting (Wijmenga/Weersma/Franke/Swertz groups) 3226-314 ERIBA Auditorium
12:00 - 17:00 Finish experimental design / (validation) plan flex desks
17:00 Deadline: have experimental design / (validation) plan approved by your supervisor(s) TBA

From Tuesday 09-02-2016 onwards

  • Regular meetings
  • Execute your experiment / plan

Week 6

From Monday 07-03-2016 onwards

  • Regular meetings
  • Write report and prepare presentation.

Thursday 10-03-2015

When What Where
13:00 - 13:30 Project Presentation I TBA
13:30 - 14:00 Project Presentation II TBA
14:00 - 14:15 Break
14:15 - 14:45 Project Presentation III TBA
14:45 - 15:15 Project Presentation IV TBA
15:15 - 15:30 Break
15:30 - 16:00 Project Presentation V TBA
16:00 - 16:30 Project Presentation VI TBA
16:30 - 17:00 Wrap up (Morris/Pieter?) TBA

Friday 11-03-2015

When What Where
17:00 Report submission deadline Your project supervisor's mailbox

Contact

Morris Swertz coordinator m.a.swertz AT rug DOT nl
Pieter Neerincx daily supervision pieter.neerincx AT gmail DOT com